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  Protein Crystallization: Techniques, Strategies, and Tips. A Laboratory Manual

IUL Biotechnology Series, 1

by Terese M. Bergfors (Editor) 

Edition: First


OUT OF PRINT





Book Details:
  • Series: IUL Biotechnology Series

  • Volume: 1

  • Binding: Hardcover 

  • Pages: 306

  • Dimensions (in inches): 1.25 x 9.75 x 6.50

  • Publisher: International University Line 

  • Publication Date: October 15, 2002 (Second Print)

  • ISBN: 0-9636817-5-3

  • List Price: $69.95

  • Price: $69.95

 


Editor's Review, August 22, 2000
Reviewer: Terese Bergfors
This book is written for biochemists, crystallographers, graduate students, lab technicians, undergraduates or anyone else working with proteins with the intention of crystallizing them. The first part introduces the beginner to the basic techniques, materials, and parameters that affect crystallization. Those readers with previous crystallization experience can turn directly to the chapter on dynamic light scattering as a diagnostic tool, or to the five chapters devoted to choosing a crystallization strategy. Interpretation of results is probably the area in which many have their greatest difficulty so 20 color plates have been included to assist in the identification of the phenomena found in crystallization drops. The second part of the book takes up more advanced methods such as crystallization for cryo-crystallography, seeding, the use of oils, microbatch methods, and special considerations for membrane proteins. Useful tips and recommendations have been collected in an A-Z section, again with an emphasis on practical problems and questions encountered at the lab bench. The appendices contain a list of good-to-have gizmos, suppliers and sources of crystallization materials, useful web sites, and a comprehensive listing of commercially available screens Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The chapters have been contributed by 18 scientists who are actively involved in the development of methodology for protein crystallization.


 

 

Crystallization cover

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Abstract:

This book is a basic, example-based laboratory manual for scientists and students who crystallize soluble and membrane proteins. The book could be useful for wide audience. Using the book, beginners and students find the practical direction how to start; intermediates can improve their techniques; experts can find advisable hints for creating new methods. The book includes three parts. The first part covers the essential techniques with emphasis on beginnersí problems. The second part contains the most up-to-date practical protocols, techniques, and tools. The third part encompasses an A-Z problem- solving guide, recipes, screens, and expertsí tips. Laboratory exercises, for use in practical courses in protein crystallization, are included.

     Written by the best experts in protein crystallization from different countries.

      Edited by Terese M. Bergfors, Dept. of Molecular Biology, Uppsala University, Sweden.

 


 

Reviews


  Reviewer: Prof. Bror Strandberg, Member of Sir John Kendrew's research team when determining the first 3D structure of protein (myoglobin)

  Crystallization has been and still is the most difficult bottleneck in macromolecular crystallography. However, during the last 40 years a huge amount of experience and knowledge has accumulated, which has made the crystallization procedures more controllable. This book describes in an elegant way all the techniques and strategies now used for crystallizing macromolecules. The book is a practical guide with the most recent protocols, recipes, and crys-tallization screens. A large number of valuable examples are included as well as important tips and a useful A-Z sec-tion. Finally, the difficult problem of 'badly behaved' macro-molecules is discussed as well as the various modifications necessary for making these macromolecules crystallizable. Protein Crystallization is an authoritative book with chapters written by well-known scientists. The book is an invaluable guide both for beginners and for the more experienced crystallizers of biological macromolecules.