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  Protein Crystallization

IUL Biotechnology Series, 8

by Terese M. Bergfors (Editor) 

Edition: Second







Book Details:
  • Series: IUL Biotechnology Series

  • Volume: 8

  • Binding: Hardcover 

  • Pages: 504

  • Dimensions (in inches): 1.20 x 9.75 x 6.50

  • Publisher: International University Line 

  • Publication Date: April 5, 2009

  • ISBN: 978-0-9720774-4-6

  • List Price: $109.95

  • Price: $109.95

 


Editor's Review, March 15, 2009
Reviewer: Terese Bergfors
Obtaining good quality protein crystals still remains a bottleneck in structural determination by X-ray crystallography. This book is written for biochemists, crystallographers, graduate students, technicians, undergraduates or anyone else working with proteins with the intention of crystallizing them. The approach is a practical one and no previous experience of the laboratory techniques is assumed. It is suitable for beginners in the field, but the extent of the material provides a thorough coverage of the topic for experienced structural biologists.
   If you already have the first edition of Protein Crystallization, why should you buy this second edition? The first edition was published in 1999. In the ten years since then, the field of protein crystallization has experienced rapid development and change.  The second edition of the book contains ten new chapters and the other seven have been revised and updated. The chapters have been written by twenty-three scientists who are actively involved in the development of methodology for protein crystallization. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the "Practical Protein Crystallization" courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere.
   The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench.


 

 

Crystallization2 cover

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Look inside this book

 

Abstract:
Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best- selling 1st edition. Completely new chapters on high-through- put methods, mass spectrometry, microcalorimetry, counter- diffusion, heavy-atom derivatization, selenomethionine- labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, char- acterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Thirty full-color plates for evaluating crys- tallization drops. Separate section of laboratory exercises, ideal for crystallization courses. AZ glossary.

     Written by the best experts in protein crystallization from different countries.

      Edited by Terese M. Bergfors, Dept. of Molecular Biology, Uppsala University, Sweden.

 


 

Reviews


  Reviewer: Dr. Herbert A. Hauptman, Nobel Laureate in Chemistry


  Why is crystallization important? At different times, different aspects of protein crystallography have assumed the role of bottleneck. Thus at one time the bottleneck consisted of the lack of a methodology for structure determination, even if a sufficient number of diffraction intensitities were available for structure solution. With the advent of powerful methods for structure solution, the bottleneck shifted to the difficulty in obtaining crystals in sufficient number and of suitable quality to do the diffraction experiment. The present book is dedicated to the problem of describing current methods for protein crystallization and describing them as clear laboratory protocols.



Reviewer: Dr. William L. Duax, American Crystallographic Association, Chief Executive Officer



This is an essential handbook for anyone engaged in crystallization of macromolecules. It is exceptionally well organized and illustrated and has contributions from all the leaders in what continues to be a challenging and critically important field.