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  Protein Crystallization Strategies for Structural Genomics

IUL Biotechnology Series, 7

by Naomi E. Chayen (Editor) 

Edition: First



Book Details:
  • Series: IUL Biotechnology Series

  • Volume: 7

  • Binding: Hardcover 

  • Pages: 290

  • Dimensions (in inches): 1.25 x 9.5 x 6.50

  • Publisher: International University Line 

  • Publication Date: July, 2007

  • ISBN-10: 0-9720774-3-X

  • ISBN-13:  978-0-9720774-3-9
  • List Price: $109.95

  • Price: $109.95

Editorial Reviews
In review Book News, Inc.®, Portland, OR

Raymond C. Stevens, Professor, The Scripps Research Institute, La Jolla, California
During the 1980s and early 1990s, we observed a tremendous breakthrough in our understanding of the physical chemistry of macromolecular crystallization due to the pioneering efforts of several leaders in the field. However, the determination of protein 3-dimensional structure was still a slow process, often taking years to complete. In the mid to late 1990s, a new renaissance of protein crystallography occurred with the development of increased throughput nanovolume crystallization robotic methods, microfluidic counter diffusion, creative screens and optimization techniques, crystal imaging, and novel crystallization methods for the very challenging membrane proteins and large macromolecular complexes. In the fast paced world of high throughput protein crystallization, this work was established by many of the authors in this book and the areas are comprehensively covered. Protein Crystallization Strategies for Structural Genomics will be highly valuable to those experimentalist interested in understanding, reproducing, or expanding upon the recent innovations. It will be an essential reference for the many small and medium size laboratories that are starting or are planning to adopt these systems and approaches. The clarity, thoroughness, and simplicity of the chapters are impressive. The best analogy for this compendium is the early days of gene sequencing when technology development was critical in moving radioactive gel sequencing to fluorescent capillary automated methods. This book is the beginning of a similar movement, initiating the vision of rapid macromolecular structure determination for providing a deeper understanding of the molecular science of life. What this book accomplishes and that many other similar books miss are the critical details necessary to understand, and further expand upon protein crystallization strategies for structural biology.



Protein Crystallization Strategies for Structural Genomics

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    The past five years have seen some of the greatest achievements in the field of protein crystallization. It is now feasible to screen thousands of potential crystallization conditions by dispensing trials consisting of nanoliter volumes in a high-throughput mode. This has cut the time of setting up experiments from weeks to minutes, a scenario that was unimaginable a few years ago. Even more incredible, is the revelation that diffracting crystals can be produced from protein samples in volumes as small as 5–20 nanoliter. The subsequent phase of image capture and analysis of the crystallization drops is also progressing in great strides.

Surprisingly, in spite of the impressive advances accomplished, the crystallization problem has not been solved. High throughput has not yet resulted in high output and the current challenge is to design new and improved techniques (of screening and optimization) for the production of useful crystals. Scientists worldwide have taken on the challenge by tackling the crystallization problem from a variety of different aspects.

Research advances in recent years have opened up the scope for the development of new methods and tools to overcome the bottleneck of protein crystallization. A variety of parameters that could previously not be explored are now accessible thanks to sophisticated apparatus and the development of new science-based techniques to monitor and control the process of crystallization. However, in order to become useful to the structural genomics effort, it is vital to miniaturize and automate these techniques and adapt them to cope with the vast numbers of “leads” resulting from the high-throughput screening procedures. Such efforts are those of the immediate future and the focus of this book.